The working group -imics /Borth at the Department of Biotechnology is looking to fill several positions: Enthusiastic lab members wanted!
Post Doc, Dissertation(s), TechnicianImprovement of Chinese Hamster Ovary Cell CHO platform technology: Cell line optimisation and engineering
Objective:
• Development of tunable gene expression toolbox
• Establishment of a stable host cell line
• Controlled induction of a high productivity phenotype
Current problems in mammalian cell line development
• CHO Cell lines are phenotypically and physiologically unstable due to high number of chromosomal rearrangements
• Host cell optimisation difficult, as properties are lost upon transfection
• Random transfection induces more uncontrollable genomic changes
• Labour and time intensive screening required
• Development times for cell lines: 4-8 months
To do´s
• Host cell line with higher genomic stability
• Targeted integration toolbox
• Control over cellular properties
Wanted
• Enthusiastic people, who want to work in a group at the forefront of CHO-research
• Independent thinkers who like to discuss new ideas and work on their realisation
• Interested individuals who want to learn cell culture technology, bioinformatics and cell engineering
START: January 2012
If you are interested, please contact Nicole Borth, DBT 3rd floor, 47564 6232 or
nicole.borth@boku.ac.at