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Development of GFP-based Technologies for the Improvement of industrial Enzymes
Diplom/Masterarbeit
Angebot online seit:
Mi 8 Aug 2018
Arbeitsort:
Kein Ort angegeben
Firma/Kontakt:
Peter Herzog
peter.herzog@boku.ac.at
 
 










MAKE ENZYMES GREAT AGAIN!


Duration: 6 months (additional months can be payed)
Begin: now!
Contact: peter.herzog@boku.ac.at, clemens.peterbauer@boku.ac.at


You bring:
• Motivation and team spirit
• Organized working attitude
• Practical experience in biochemistry & molecular biology


You get:
• Relaxed working atmosphere
• Participation in frontline science
• Possibility to get published
• Possibility to work abroad collaborators: MIT, RWTH


Work package:
• Molecular cloning
• Recombinant expression
• Protein purification
• Yeast surface display
• Immobilization techniques
• Flow cytometry & FACS


Protein engineering is a vital technique to deminish disadvantages and strengthen advantages of industrial enzymes. Here, the development of novel technology is very impactful for the success of new enzymatic processes.


In 2014, the GFP-derived fusion protein roGFP2-Orp1 was introduced as a sensor to very sensitively respond to H2O2 by changing its fluorescence.


This project aims at developing technology to allow using roGFP2-Orp1 as a sensor for the detection of enzymatic H2O2 production. With this, we seek to realize enzyme engineering platforms that work on the level of individual cells. The combination with yeast surface display will allow to employ FACS as a read-out tool for enzyme activity and will greatly simplify the discovery of enhanced enzymes.