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Establishment of an inducible silencing system to study the fungal histone variant H2A.Z
Diplom/Masterarbeit
Angebot online seit:
So 13 Sep 2020
Arbeitsort:
Kein Ort angegeben
Firma/Kontakt:
anna.atanasoff-kardjalieff@boku.ac.at
 
 










I am looking for a motivated master student in the field of molecular biology, who is interested to work with a fungal plant pathogen!


Fusarium fujikuroi belongs to one of the economically most notable phytopathogens worldwide, causing the notorious ‘bakanae’ disease on rice seedlings. During the infection process noxious metabolites are synthesized and released by the fungus, impairing plants health and threatening food security. To develop sustainable and efficient strategies to constrain fungal plant diseases it is important to understand the underlying mechanisms influencing their gene expression. In all eukaryotes DNA-dependent processes, depends on the chromatin landscape and its modification. In this work we will study, the histone variant H2A.Z, which is believed to modify chromatins availability. Since H2A.Z is essential in Fusarium species, we will establish the inducible Tet-On and Tet-Off system to study the influence of the histone variant on fungal development, secondary metabolism and virulence and draw a comparison of both systems applicability.


We are looking for:

• Student familiar in the field of Biotechnology, Microbiology, Biochemistry or relating studies

• Highly motivated student who is curious of working with plant pathogens

• Ability to work independently under supervision


We offer:

• Modern infrastructure and a very friendly working environment ;)

• Learning of various techniques including

• In silico cloning

•Fungal phenotyping

• Western Blot, RT-qPCR

• Rice infections etc….

• By the successful complementation to be part of a scientific publication

• Travel expenses to Tulln will be refunded


Facts:


Duration: 6 months

Start: ideal October/November 2020

Location: UFT-Tulln AG Strauss


If you are interested, please send your CV to Anna Atanasoff-Kardjalieff

E-mail: anna.atanasoff-kardjalieff@boku.ac.at