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Cloning and homologous expression of hydrolases for aroma improvement of low pH food systems
Diplom/Masterarbeit
Angebot online seit:
Sa 16 Mai 2009
Arbeitsort:
Vienna, Austria
Firma/Kontakt:
Herbert Michlmayr
herbert.michlmayr@boku.ac.at
herbert.michlmayr@boku.ac.at
Tasks
· “fish” genes from exotic lactic acid bacteria like Oenococcus oeni and Oenococcus kitaharae by PCR and gene walking
· Bioinformatics: get the sequences of your genes, do orthology and homology research, cladistic interpretation, identify conserved domains... publish the sequences in a database, study PTS-system
· Expression in lactic acid bacteria (self clones) with fusion tags
· Enzyme purification and characterization, Michaelis Menten Kinetics, test transglycosylase activities
· Immobilization on non-toxic polymers
· If you want, directed evolution by site directed or random mutagenesis to improve pH stability
· Hydrolyze aroma precursors from wine or fruit juices
· Sensory evaluation, aroma profiling by GC-MS, chemical nose....
Duration:
Bachelor thesis: 2 months
Master/Diploma thesis: 6 months
Requirements:
Fundamental knowledge in molecular biology and biochemistry, lab coat
Contact: Herbert Michlmayr herbert.michlmayr@boku.ac.at
Prof. Klaus Dieter. Kulbe klaus_dieter.kulbe@boku.ac.at
Institute for Food Biotechnology, Muthgasse 18, 2nd Floor
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