Model Based Strain Engineering

 

Enhancing protein production in the yeast

P. pastoris

 

 

Pichia pastoris is THE yeast for recombinant protein production. However, further strain improvements are necessary to make this system even more competitive. The approach used in this study is the systematic knock-out of genes, predicted by a genome-scale metabolic model that was developed in a cooperation with Prof. Sang Yup Lee from the Korea Advanced Institute of Science and Technology.

 

The start of the project will be at the beginning of 2011.

 

 

The work will include

 

Cloning of vectors in E. coli

Generation and screening of P. pastoris knock-out strains

Product analysis and quantification via SDS-PAGE, Western Blot or ELISA

Characterisation of the engineered strains (productivity, enzymatic assays, fermentation,...)

 

 

Please Contact

 

Martin Pfeffer

Tel.: (+43)1/47654 – 6656

e-mail: martin.pfeffer@boku.ac.at