This quantitative trait locus (QTL) encodes a gene that has a large effect on the overall resistance against the fungal pathogen Fusarium graminearum. We would like to identify and characterize this gene.
Therefore, you will contribute to
- generating genome locus specific markers (PCRs, mostly)
- screening and sequencing BAC clones harboring this piece of
genomic sequence
- using virus-induced gene silencing to temporarily silence
candidate genes to test whether they have an effect on the resistance of Fusarium-challenged wheat heads
The practical work will be carried out in our laboratories/glass houses in Tulln (20 min from Vienna). Basic molecular biology skills (DNA/RNA-prep, molecular cloning, PCR) are advantageous but not mandatory. Start immediately or at your earliest convenience.