Background:
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by RNA interference. They have been identified to target genes of virtually every cellular pathway, and have recently been established as important tools in diagnosis and treatment of disease, as well as cell engineering strategies.

Aim:
The aim of this project is to implement a novel technology in our lab that allows rapid flow-cytometry based quantification of microRNA expression in CHO cells. This technology will then be used in collaboration with an industrial partner to identify microRNAs that correlate with specific cellular properties that define an “excellent” recombinant CHO cell line.

Techniques:
Standard cell culture, molecular biology and bioinformatic techniques including (but not limited to):
1) Mammalian cell culture (Transfection / Flow Cytometry / Batch Cultivation)
2) RNA Work: RNA Purification & Quality Control / quantitative PCR / flow-based quantification
3) Data analysis: Analysis of microRNA expression data (R, Genesis, etc.)
SOP Development

Start: 06/2013
Duration: 6 months

If you have experience in molecular biology, are highly motivated to implement a novel technology in one of the hottest areas of industrial biotechnology, and want to find out more, please contact:

Dr. Matthias Hackl
T: 01 47654 6803
E: matthias.hackl@boku.ac.at