Master thesis:

Protein A Affinity Chromatography is the standard purification method for monoclonal antibodies. Constantly, novel Protein A affinity resins with improved performance are developed by various companies. The goal of this work is to test a novel Protein A resin and perform a benchmark study against the best performing resins available on the market. The study comprises determination of dynamic binding capacities, elution behavior and removal of host cell proteins from cell culture supernatants. In the course of the study a complete laboratory reference purification process for IgG comprising of a Protein A affinity step, a cation exchange and an anion exchange step should also be established. For this process, novel high capacity ion exchange membrane absorbers should be tested and implemented. Process optimization with respect to highest purity, throughput and productivity should further be accomplished.

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