Golden Gate cloning is based on the use of type IIs endonucleases that cleave DNA outside of their recognition site. The design of the overhang sequences –also called fusion sites -enables fast and variable directional assembly of multiple fragments.

In a previous master thesis, the golden gate cloning technique was successfully adapted and established for strain engineering applications in Pichia pastoris. The new diploma/master thesis will focus on further methodical improvements and on the cloning of selected sequences to improve recombinant protein production.

The work will include:
- Planning of cloning strategy, application for strain engineering of P. pastoris
- Efficiency testing and development of strategies for further improvement
- Small scale screenings and analytical methods such as qPCR, ELISA and flow cytometry

We are looking for a highly motivated and ambitious Diploma/Master Student who wants to join our group and work on this topic!

Start:February/March 2015

If you are interested please contact:

Priv. Doz. Dr. Brigitte Gasser
Tel.: (+43) 1 / 47654-6813
e-mail: brigitte.gasser@boku.ac.at

Roland Prielhofer (6810)
e-mail: roland.prielhofer@boku.ac.at