Information:

The application of microchip technology for clinical diagnostics or personalized medicine provides the opportunity to develop the next generation of cell analysis devices with improved performance, fast operation times and low production costs.

Goal:

We are developing a device capable of capturing T- and B-cells selectively from peripheral blood mononuclear cells - PBMC´s by combining microfluidics with recombinant S-layer protein SbpA/ZZ. With its antifouling properties, and attached antibodies on the Fc-specific binding domain the cell retention in laminar flow is performed in a unique way.
Your work includes the validation of microfluidic setups and devices to reproduce and identify ratios of peripheral blood mononuclear cells (PBMCs) by on chip phenotyping for diagnostic applications.

Methods:

-PBMCs isolation
-Flow Cytometry
-Cell chip fabrication
-S-layer technology
-Fluorescence staining on chip and confocal microscopy

Contact:

Institut für Synthetische Bioarchitekturen
Dr. Seta Küpcü.
Email: seta.kuepcue@boku.ac.at
Tel: (+43) 1 / 47654-2215

Department für Nanobiotechnology
Muthgasse 11/II
1190 Wien