The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. Originally derived from bacteria, its use is now well established in a lot of different organisms.

The diploma/master thesis will focus on the adaption and optimization of the CRISPR/Cas9 system for the generation of knockout strains and the introduction of other mutations into the genome of the yeast Pichia pastoris.

The work will include:

Planning of cloning strategy using advanced methods

Testing of the method in P. pastoris and application for selected target genes

Determination of the mutation efficiency and development of strategies for improvement

We are looking for a highly motivated and ambitious Diploma/Master Student who wants to join our group and work on this topic!

Start: November/December 2015

Where: in the group of Prof. Mattanovich at the Department of Biotechnolgy